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VitroView Congo Red Amyloid Stain Kit (#VB-3011)



Congo Red is a histological stain that has been used to detect the presence of amyloid deposits, including those associated with Alzheimer’s disease. The stain binds to beta-pleated sheet structures, which are characteristic of amyloid fibrils. Therefore, Congo Red staining can be used to visualize beta-amyloid plaques in the brain tissue of individuals with Alzheimer’s disease.

Congo Red Amyloid Stain Kit is used for the detection of amyloid in formalin-fixed, paraffin-embedded tissue sections with amyloidosis, and may be used for frozen sections as well. Amyloid deposits will stain red, while nuclei will stain blue. The thickness of sections is usually 5 µm. However, in cases containing low amyloid deposits, 10µm thick sections will give better results.


Kit Contents

  •    VB-3011-1 Congo Red Solution ——————–250 ml
  •    VB-3011-2 Alkaline Alcohol Solution ————250 ml
  •    VB-3011-3 Mayer’s Hematoxylin Solution —- 250 ml


Storage Condition:

Store at room temperature.



  • For paraffin-embedded tissue sections, deparaffinize and hydrate to 70% alcohol. Rinse in distilled water. For frozen sections, dry sections for 5 minutes. Fix sections in neutral formalin solution for 20 minutes. Rinse in distilled water.
  • Stain in congo red solution for 15-20 minutes.
  • Rinse in distilled water.
  • Differentiate quickly (5-10 dips) in alkaline alcohol solution.
  • Rinse in tap water for 1 minute.
  • Counterstain with Mayer’s hematoxylin for 30 seconds.
  • Rinse in tap water for 2 minutes.
  • Dehydrate through 95% alcohol, 2 changes of 100% alcohol, 3 minutes each.
  • Clear with 3 changes of xylene (5 minute per change)
  • Mount coverslip onto glass slide with Permount or some other suitable organic mounting medium.



  • Amyloid, elastic fibers, eosinophil granules ——– red
  • Nuclei —————————————————————- blue


 Positive Controls

Know amyloidosis containing tissue (i.e. Alzheimer’s disease).


Note: This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Precautions: Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.

VitroView Congo Red Amyloid Stain Kit (#VB-3011)

SKU: VB-3011
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