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VitroView Mammary Gland Whole Mount Stain Kit(#VB-3001)

Storagestored at 4℃



Wholemount staining of mouse mammary glands shows the structure of the whole gland and how it is altered in diseased states and in response to various hormones, or carcinogens. VitroView™ Mammary Gland Whole Mount Stain Kit is very useful for the study of mammary gland development.


Kit Contents

  •      VB-3001-1  Carnoy’s fixative Solution—————-250ml
  •      VB-3001-2  Carmine Alum Staining Solution——-250ml

 Storage Condition


      If stored at 4 oC, the kit can be used for several months.  Discard when color becomes weak.


 Material Needed But NOT Supplied with the Kit

  •  Staining jar
  • Glass slides
  • Ethanol
  • Xylene
  • Mount media



  • Mammary gland tissues are spread out on glass slide.
  • Fix in Carnoy’s fixative for 2 to 4 hours at room temperature.
  • Wash in 70 % Ethanol for 30 min. Change gradually to distilled water
  • Rinse in distilled water for 5 min.
  • Stain in carmine alum staining solution overnight at room temperature.
  • Wash in 70 % Ethanol  for 15 min
  • Wash in 95 % Ethanol for 15 min
  • Wash in 100 % Ethanol for 15 min
  • Clear with 3 changes of xylene (10 minute per change)
  • Mount coverslip onto glass slide with Permount or some other suitable organic mounting medium. 


Note: Carnoy’s Fixative Solution and Carmine Alum Staining Solution can be reused.


After photographic documentation the tissue can be embedded in paraffin for sectioning and conventional histological staining.

  • Immerse slide into xylene to remove mounting medium
  • Change xylene 2 times
  • Transfer to 1:1 xylene:paraffin (60oC)
  • Change xylene 2 times and embed in paraffin


Note: This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Precautions: Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.

VitroView Mammary Gland Whole Mount Stain Kit (#VB-3001)

SKU: VB-3001
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