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VitroView Oil Red O Stain Kit (#VB-3007)



Oil Red O is a lysochrome diazo dye used for detecting neutral triglycerides and lipids in tissueIt is performed on fresh frozen tissue sections or cultured cells. Oil Red O Stain Kit is used for lipid and fat staining on formalin fixed frozen sections. It is not suitable for paraffin embedded tissue sections.


Kit Contents

  • VB-3007-1 Pre-Stain Solution ———————– 100 ml
  • VB-3007-2 Oil Red O Solution ————————100 ml
  • VB-3007-3 Differentiation Solution————— 100 ml
  • VB-3007-4 Mayer’s Hematoxylin Solution —- 100 ml



Room temperature.



  • Cut frozen sections to 8 to10μm. Air dry the sections to the slides
  • Fix in 10% buffered formalin for 20 mins at room temperature, briefly wash with running tap water for 3 mins.
  • Place in Pre-Stain Solution for 2 mins
  • Stain in pre-warmed Oil Red O solution for 10 minutes at room temperature
  • Differentiate in Differentiation Solution for 5 minutes at room temperature
  • Rinse in 2 water changes of distilled at room temperature.
  • Lightly stain nuclei with Mayer’s Hematoxylin Solution for 1 minute.
  • Rinse with tap water for 3 mins
  • Mount with aqueous mounting medium.


Technical Points

  • Include a positive control
  • Cryostat sections 8 to 10 microns for formalin fixed smears. Formalin fixation gives good results.
  • Step 9 – Obviously, the section must not be taken through clearing solvents prior to mounting, as this will remove the lipid to be detected.



  • Lipid…………………………….. red
  • Nuclei…………………………….blue


Note: This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Precautions: Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.

VitroView Oil Red O Stain Kit (#VB-3007)

SKU: VB-3007
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