Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP.
This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
Application
Dilution
Western Blotting
1:1000
Immunoprecipitation
1:100
Immunohistochemistry (Paraffin)
1:400 - 1:1600
Immunofluorescence (Immunocytochemistry)
1:400 - 1:1600
Flow Cytometry
1:400 - 1:1600
Chromatin IP
1:100
Chromatin IP-seq
1:100
CUT&RUN
1:100